CBX5 antibody (pSer92)

Catalog No. ABIN7220722

The Rabbit Polyclonal anti-CBX5 antibody (ABIN7220722) specifically detects CBX5 in ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CBX5 antibody (pSer92) (ABIN7220722)

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBX5 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CBX5 Antibody

Binding Specificity: pSer92

Purpose: Rabbit Anti-HP1α (phospho Ser92) Polyclonal Antibody

Specificity: Phospho-HP1α (S92) Polyclonal Antibody detects endogenous levels of HP1α protein only when phosphorylated at S92.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human HP1alpha Phospho-Ser92

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CBX5

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Alternative Name: HP1alpha

Background: CBX5, HP1A, Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alphaCBX5 encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The chromobox 5 is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Gene ID: 23468

UniProt: P45973