Nesprin3 antibody (AA 140-220)

Catalog No. ABIN7219964

The Rabbit Polyclonal anti-Nesprin3 antibody (ABIN7219964) specifically detects Nesprin3 in WB, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for Nesprin3 antibody (AA 140-220) (ABIN7219964)

Target: Nesprin3 (C14orf49) (Chromosome 14 Open Reading Frame 49 (C14orf49))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nesprin3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-Nesprin3 Antibody

Binding Specificity: AA 140-220

Purpose: Rabbit Anti-Nesprin-3 Polyclonal Antibody

Specificity: Nesprin-3 Polyclonal Antibody detects endogenous levels of Nesprin-3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Nesprin-3 at AA range: 140-220

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Nesprin3

Target: Nesprin3 (C14orf49) (Chromosome 14 Open Reading Frame 49 (C14orf49))

Alternative Name: Nesprin-3

Background: SYNE3, C14orf49, Nesprin-3, Nuclear envelope spectrin repeat protein 3Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.

Molecular Weight: 115kD

Gene ID: 161176

UniProt: Q6ZMZ3

Pathways: Maintenance of Protein Location