Clusterin antibody (AA 370-450)

Catalog No. ABIN7214254

The Rabbit Polyclonal anti-Clusterin antibody is suitable to detect Clusterin in samples from Human, Rat and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for Clusterin antibody (AA 370-450) (ABIN7214254)

Target: Clusterin (CLU)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Clusterin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Clusterin Antibody

Binding Specificity: AA 370-450

Purpose: Rabbit Anti-Clusterin Polyclonal Antibody

Specificity: Clusterin Polyclonal Antibody detects endogenous levels of Clusterin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Clusterin at AA range: 370-450

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Clusterin

Target: Clusterin (CLU)

Alternative Name: Clusterin

Background: CLU, APOJ, CLI, KUB1, AAG4, Clusterin, Aging-associated gene 4 protein, Apolipoprotein J, Apo-J, Complement cytolysis inhibitor, CLI, Complement-associated protein SP-40, 40, Ku70-binding protein 1, NA1/NA2, Testosterone-repressed prostate mClusterin encoded by CLU is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.

Molecular Weight: 52kD

Gene ID: 1191

UniProt: P10909

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling