Cofilin antibody (AA 30-110)

Catalog No. ABIN7214276

The Rabbit Polyclonal anti-Cofilin antibody is suitable to detect Cofilin in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IF.

Quick Overview for Cofilin antibody (AA 30-110) (ABIN7214276)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cofilin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-Cofilin Antibody

Binding Specificity: AA 30-110

Purpose: Cofilin Polyclonal Antibody

Specificity: Cofilin Polyclonal Antibody detects endogenous levels of Cofilin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Cofilin at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: Cofilin

Background: Rabbit Anti-Cofilin Polyclonal Antibody,CFL1, CFL, Cofilin-1, 18 kDa phosphoprotein, p18, Cofilin, non-muscle isoform, CFL2, Cofilin-2, Cofilin, muscle isoform,CFL2 encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. Cofilin 2 is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH -dependent manner. Mutations in CFL2 cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants.,Cofilin-1

Molecular Weight: observerd band 19kDa

Gene ID: 1073, 1072

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events