CKM antibody (AA 10-90)

Catalog No. ABIN7214322

The Rabbit Polyclonal anti-CKM antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect CKM in samples from Human, Mouse and Rat.

Quick Overview for CKM antibody (AA 10-90) (ABIN7214322)

Target: CKM (Creatine Kinase, Muscle (CKM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CKM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CKM Antibody

Binding Specificity: AA 10-90

Purpose: Creatine Kinase M Polyclonal Antibody

Specificity: Creatine Kinase M Polyclonal Antibody detects endogenous levels of Creatine Kinase M protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human Creatine Kinase M at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CKM

Target: CKM (Creatine Kinase, Muscle (CKM))

Alternative Name: Creatine Kinase M

Background: Rabbit Anti-Creatine Kinase M Polyclonal Antibody,CKM, CKMM, Creatine kinase M-type, Creatine kinase M chain, M-CK,Creatine kinase, M-typeencoded by CKM is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.,Creatine kinase M-type

Molecular Weight: observerd band 43kDa

Gene ID: 1158

UniProt: P06732