CUBN antibody (N-Term)

Catalog No. ABIN7225140

The Rabbit Polyclonal anti-CUBN antibody is suitable to detect CUBN in samples from Human, Rat and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for CUBN antibody (N-Term) (ABIN7225140)

Target: CUBN (Cubilin (Intrinsic Factor-Cobalamin Receptor) (CUBN))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CUBN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CUBN Antibody

Binding Specificity: N-Term

Purpose: Rabbit Anti-Cubilin Polyclonal Antibody

Specificity: Cubilin Polyclonal Antibody detects endogenous levels of Cubilin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Cubilin

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CUBN

Target: CUBN (Cubilin (Intrinsic Factor-Cobalamin Receptor) (CUBN))

Alternative Name: Cubilin

Background: CUBN, IFCR, Cubilin, 460 kDa receptor, Intestinal intrinsic factor receptor, Intrinsic factor-cobalamin receptor, Intrinsic factor-vitamin B12 receptorCubilin (CUBN) acts as a receptor for intrinsic factor-vitamin B12 complexes. The role of receptor is supported by the presence of 27 CUB domains. Cubulin is located within the epithelium of intestine and kidney. Mutations in CUBN may play a role in autosomal recessive megaloblastic anemia.

Molecular Weight: 400kD

Gene ID: 8029

UniProt: O60494

Pathways: Metabolism of Steroid Hormones and Vitamin D, Lipid Metabolism