Cyclin B1 antibody (pSer147)

Catalog No. ABIN7225714

The Rabbit Polyclonal anti-Cyclin B1 antibody (ABIN7225714) specifically detects Cyclin B1 in WB, ELISA and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Cyclin B1 antibody (pSer147) (ABIN7225714)

Target: Cyclin B1 (CCNB1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cyclin B1 Antibody

Binding Specificity: pSer147

Purpose: Cyclin B1 (phospho Ser147) Polyclonal Antibody

Specificity: Phospho-Cyclin B1 (S147) Polyclonal Antibody detects endogenous levels of Cyclin B1 protein only when phosphorylated at S147.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Cyclin B1 Phospho-Ser147

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1

Background: Rabbit Anti-Cyclin B1 (phospho Ser147) Polyclonal Antibody,CCNB1, CCNB, G2/mitotic-specific cyclin-B1,The protein encoded by CCNB1 (cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.,cyclin-B1

Molecular Weight: observerd band 60kDa

Gene ID: 891

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase