Cyclin E2 antibody (pThr392)

Catalog No. ABIN7225830

This anti-Cyclin E2 antibody is a Rabbit Polyclonal antibody detecting Cyclin E2 in IF and ELISA. Suitable for Human and Mouse.

Quick Overview for Cyclin E2 antibody (pThr392) (ABIN7225830)

Target: Cyclin E2 (CCNE2)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin E2 antibody is un-conjugated

Application: Immunofluorescence (IF), ELISA

Product Details anti-Cyclin E2 Antibody

Binding Specificity: pThr392

Purpose: Cyclin E2 (phospho Thr392) Polyclonal Antibody

Specificity: Phospho-Cyclin E2 (T392) Polyclonal Antibody detects endogenous levels of Cyclin E2 protein only when phosphorylated at T392.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Cyclin E2 Phospho-Thr392

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Cyclin E2

Target: Cyclin E2 (CCNE2)

Alternative Name: Cyclin E2

Background: Rabbit Anti-Cyclin E2 (phospho Thr392) Polyclonal Antibody,CCNE2, G1/S-specific cyclin-E2,The protein encoded by CCNE2 (cyclin E2)belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of CCNE2 peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells.,G1

Gene ID: 9134

UniProt: O96020

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases