Cyclin F antibody (AA 710-790)

Catalog No. ABIN7214398

The Rabbit Polyclonal anti-Cyclin F antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect Cyclin F in samples from Human.

Quick Overview for Cyclin F antibody (AA 710-790) (ABIN7214398)

Target: Cyclin F (CCNF)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin F antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cyclin F Antibody

Binding Specificity: AA 710-790

Purpose: Cyclin F Polyclonal Antibody

Specificity: Cyclin F Polyclonal Antibody detects endogenous levels of Cyclin F protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human Cyclin F at AA range: 710-790

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Cyclin F

Target: Cyclin F (CCNF)

Alternative Name: Cyclin F

Background: Rabbit Anti-Cyclin F Polyclonal Antibody,CCNF, FBX1, FBXO1, Cyclin-F, F-box only protein 1,CCNF encodes a member of the cyclin family. Cyclins are important regulators of cell cycle transitions through their ability to bind and activate cyclin-dependent protein kinases. This member also belongs to the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. Cyclin F encoded by CCNF belongs to the Fbxs class and it was one of the first proteins in which the F-box motif was identified.,Cyclin-F

Molecular Weight: observerd band 88kDa

Gene ID: 899

UniProt: P41002