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Cyclin F antibody

CCNF Reactivity: Human WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN265381
  • Target See all Cyclin F (CCNF) Antibodies
    Cyclin F (CCNF)
    Reactivity
    • 40
    • 16
    • 8
    Human
    Host
    • 38
    • 2
    Rabbit
    Clonality
    • 39
    • 1
    Polyclonal
    Conjugate
    • 13
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Cyclin F antibody is un-conjugated
    Application
    • 37
    • 21
    • 13
    • 13
    • 5
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody detects endogenous levels of Cyclin F protein (region surrounding Ile772).
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen
    Purity
    > 95 % pure by SDS-PAGE
    Top Product
    Discover our top product CCNF Primary Antibody
  • Application Notes
    ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    Cyclin F (CCNF)
    Alternative Name
    Cyclin F (CCNF Products)
    Synonyms
    Cyclin-F antibody, DKFZp459P0432 antibody, FBX1 antibody, FBXO1 antibody, CycF antibody, Fbxo1 antibody, cb443 antibody, zgc:77240 antibody, cyclin F antibody, cyclin F L homeolog antibody, CCNF antibody, ccnf.L antibody, ccnf antibody, Ccnf antibody
    Background
    Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins.Synonyms: CCNF, Cyclin-F, G2/mitotic-specific cyclin-F
    Molecular Weight
    approx. 88 kDa
    Gene ID
    899
    NCBI Accession
    NP_001752
    UniProt
    P41002
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