Cyclin F antibody (AA 710-790)

Catalog No. ABIN7214398

The Rabbit Polyclonal anti-Cyclin F antibody is suitable to detect Cyclin F in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for Cyclin F antibody (AA 710-790) (ABIN7214398)

Target: Cyclin F (CCNF)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin F antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Cyclin F Antibody

Binding Specificity: AA 710-790

Purpose: Rabbit Anti-Cyclin F Polyclonal Antibody

Specificity: Cyclin F Polyclonal Antibody detects endogenous levels of Cyclin F protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Cyclin F at AA range: 710-790

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Cyclin F

Target: Cyclin F (CCNF)

Alternative Name: Cyclin F

Background: CCNF, FBX1, FBXO1, Cyclin-F, F-box only protein 1CCNF encodes a member of the cyclin family. Cyclins are important regulators of cell cycle transitions through their ability to bind and activate cyclin-dependent protein kinases. This member also belongs to the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. Cyclin F encoded by CCNF belongs to the Fbxs class and it was one of the first proteins in which the F-box motif was identified.

Molecular Weight: 88kD

Gene ID: 899

UniProt: P41002