Cytochrome b5 (CYTB5) (AA 30-110) antibody

Catalog No. ABIN7214472

The Rabbit Polyclonal anti-Cytochrome b5 antibody (ABIN7214472) specifically detects Cytochrome b5 in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Cytochrome b5 (CYTB5) (AA 30-110) antibody (ABIN7214472)

Target: Cytochrome b5 (CYTB5)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details

Binding Specificity: AA 30-110

Purpose: Rabbit Anti-Cytochrome b5 Polyclonal Antibody

Specificity: Cytochrome b5 Polyclonal Antibody detects endogenous levels of Cytochrome b5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Cytochrome b5 at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Cytochrome b5 (CYTB5)

Alternative Name: Cytochrome b5

Background: CYB5A, CYB5, Cytochrome b5, Microsomal cytochrome b5 type A, MCB5Cytochrome b5 type A encoded by CYB5A is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in CYB5A are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for CYB5A.

Molecular Weight: 15kD

Gene ID: 1528

UniProt: P00167