COX5B antibody (AA 30-110)

Catalog No. ABIN7219578

This Rabbit Polyclonal antibody specifically detects COX5B in ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for COX5B antibody (AA 30-110) (ABIN7219578)

Target: COX5B (Cytochrome C Oxidase Subunit Vb (COX5B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COX5B antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-COX5B Antibody

Binding Specificity: AA 30-110

Purpose: Rabbit Anti-COX5b Polyclonal Antibody

Specificity: COX5b Polyclonal Antibody detects endogenous levels of COX5b protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human COX5b at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for COX5B

Target: COX5B (Cytochrome C Oxidase Subunit Vb (COX5B))

Alternative Name: COX5b

Background: COX5B, Cytochrome c oxidase subunit 5B, mitochondrial, Cytochrome c oxidase polypeptide VbCytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. COX5B encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.

Gene ID: 1329

UniProt: P10606