DDX20 antibody (AA 250-330)

Catalog No. ABIN7219280

The Rabbit Polyclonal anti-DDX20 antibody is suitable to detect DDX20 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for DDX20 antibody (AA 250-330) (ABIN7219280)

Target: DDX20 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20 (DDX20))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX20 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DDX20 Antibody

Binding Specificity: AA 250-330

Purpose: Rabbit Anti-Gemin3 Polyclonal Antibody

Specificity: Gemin3 Polyclonal Antibody detects endogenous levels of Gemin3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Gemin3 at AA range: 250-330

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DDX20

Target: DDX20 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20 (DDX20))

Alternative Name: Gemin3

Background: DDX20, DP103, GEMIN3, Probable ATP-dependent RNA helicase DDX20, Component of gems 3, DEAD box protein 20, DEAD box protein DP 103, Gemin-3DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX20 encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs.

Molecular Weight: 90kD

Gene ID: 11218

UniProt: Q9UHI6

Pathways: Ribonucleoprotein Complex Subunit Organization