DDX3Y antibody (AA 10-90)

Catalog No. ABIN7225592

The Rabbit Polyclonal anti-DDX3Y antibody is suitable to detect DDX3Y in samples from Human and Mouse. It has been validated for IHC, ELISA and IF.

Quick Overview for DDX3Y antibody (AA 10-90) (ABIN7225592)

Target: DDX3Y (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, Y-Linked (DDX3Y))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX3Y antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-DDX3Y Antibody

Binding Specificity: AA 10-90

Purpose: Rabbit Anti-DDX3Y Polyclonal Antibody

Specificity: DDX3Y Polyclonal Antibody detects endogenous levels of DDX3Y protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human DDX3Y at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DDX3Y

Target: DDX3Y (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, Y-Linked (DDX3Y))

Alternative Name: DDX3Y

Background: DDX3Y, DBY, ATP-dependent RNA helicase DDX3Y, DEAD box protein 3, Y-chromosomalThe protein encoded by DDX3Y (DEAD-box helicase 3, Y-linked) is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of DDX3Y is not complemented by DDX3X. Mutations in DDX3Y result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both DDX3Y and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene ID: 8653

UniProt: O15523