DDX52 antibody (AA 530-610)

Catalog No. ABIN7214540

The Rabbit Polyclonal anti-DDX52 antibody (ABIN7214540) specifically detects DDX52 in WB, ELISA, IHC and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for DDX52 antibody (AA 530-610) (ABIN7214540)

Target: DDX52 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 52 (DDX52))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX52 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-DDX52 Antibody

Binding Specificity: AA 530-610

Purpose: Rabbit Anti-DDX52 Polyclonal Antibody

Specificity: DDX52 Polyclonal Antibody detects endogenous levels of DDX52 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human DDX52 at AA range: 530-610

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DDX52

Target: DDX52 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 52 (DDX52))

Alternative Name: DDX52

Background: DDX52, ROK1, HUSSY-19, Probable ATP-dependent RNA helicase DDX52, ATP-dependent RNA helicase ROK1-like, DEAD box protein 52DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DEAD box protein 52 (DDX52), also known as ATP-dependent RNA helicase ROK1-like or HUSSY-19, is a 599 amino acid protein belonging to the DEAD box helicase family. Localized to the nucleus, DDX52 is phosphorylated by ATM or ATR upon DNA damage. DDX52 contains one helicase ATP-binding domain and one helicase C-terminal domain.

Molecular Weight: 67kD

Gene ID: 11056

UniProt: Q9Y2R4