DHX8 antibody (AA 360-440)

Catalog No. ABIN7214546

This Rabbit Polyclonal antibody specifically detects DHX8 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for DHX8 antibody (AA 360-440) (ABIN7214546)

Target: DHX8 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 (DHX8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHX8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DHX8 Antibody

Binding Specificity: AA 360-440

Purpose: Rabbit Anti-DDX8 Polyclonal Antibody

Specificity: DDX8 Polyclonal Antibody detects endogenous levels of DDX8 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human DDX8 at AA range: 360-440

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DHX8

Target: DHX8 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 (DHX8))

Alternative Name: DDX8

Background: DHX8, DDX8, ATP-dependent RNA helicase DHX8, DEAH box protein 8, RNA helicase HRH1DHX8 is a member of the DEAH box polypeptide family. DEAH-box helicase 8 contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants.

Molecular Weight: 150kD

Gene ID: 1659

UniProt: Q14562