DAPK3 antibody (pThr265)

Catalog No. ABIN7219972

The Rabbit Polyclonal anti-DAPK3 antibody is suitable to detect DAPK3 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for DAPK3 antibody (pThr265) (ABIN7219972)

Target: DAPK3 (Death-Associated Protein Kinase 3 (DAPK3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAPK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-DAPK3 Antibody

Binding Specificity: pThr265

Purpose: Rabbit Anti-DAPK3 (phospho Thr265) Polyclonal Antibody

Specificity: Phospho-DAPK3 (T265) Polyclonal Antibody detects endogenous levels of DAPK3 protein only when phosphorylated at T265.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human DAPK3 Phospho-Thr265

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DAPK3

Target: DAPK3 (Death-Associated Protein Kinase 3 (DAPK3))

Alternative Name: DAPK3

Background: DAPK3, ZIPK, Death-associated protein kinase 3, DAP kinase 3, DAP-like kinase, Dlk, MYPT1 kinase, Zipper-interacting protein kinase, ZIP-kinaseDeath-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis.

Molecular Weight: 52kD

Gene ID: 1613

UniProt: O43293