Desmoglein 1 antibody (AA 30-90)

Catalog No. ABIN7228306

The Rabbit Polyclonal anti-Desmoglein 1 antibody (ABIN7228306) specifically detects Desmoglein 1 in WB, IHC, IF and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Desmoglein 1 antibody (AA 30-90) (ABIN7228306)

Target: Desmoglein 1 (DSG1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Desmoglein 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-Desmoglein 1 Antibody

Binding Specificity: AA 30-90

Purpose: Rabbit Anti-Dsg1 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of Dsg1

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human Dsg1 protein at AA range: 30-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:500-200,ELISA 1:10000-20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Desmoglein 1

Target: Desmoglein 1 (DSG1)

Alternative Name: Dsg1

Background: Desmoglein-1, Cadherin family member 4, Desmosomal glycoprotein 1, DG1, DGI, Pemphigus foliaceus antigenDSG1 encodes a member of the desmoglein protein subfamily. Desmogleins, along with desmocollins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. DSG1 is found in a cluster with other desmoglein family members on chromosome 18. The encoded protein has been identified as a target of auto-antibodies in the autoimmune skin blistering disease pemphigus foliaceus. Disruption of DSG1 has also been associated with the skin diseases palmoplantar keratoderma and erythroderma.

Molecular Weight: 160kD

Gene ID: 1828

UniProt: Q02413

Pathways: Cell-Cell Junction Organization