DGKA antibody (AA 280-360)

Catalog No. ABIN7214552

This Rabbit Polyclonal antibody specifically detects DGKA in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for DGKA antibody (AA 280-360) (ABIN7214552)

Target: DGKA (Diacylglycerol Kinase, alpha 80kDa (DGKA))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGKA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-DGKA Antibody

Binding Specificity: AA 280-360

Purpose: Rabbit Anti-DGK-α Polyclonal Antibody

Specificity: DGK-α Polyclonal Antibody detects endogenous levels of DGK-α protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human DGK-alpha at AA range: 280-360

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DGKA

Target: DGKA (Diacylglycerol Kinase, alpha 80kDa (DGKA))

Alternative Name: DGK-alpha

Background: DGKA, DAGK, DAGK1, Diacylglycerol kinase alpha, DAG kinase alpha, 80 kDa diacylglycerol kinase, Diglyceride kinase alpha, DGK-alphaDiacylglycerol kinase alpha encoded by DGKA belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

Molecular Weight: 80kD

Gene ID: 1606

UniProt: P23743