DGKD antibody (AA 10-90)

Catalog No. ABIN7214554

The Rabbit Polyclonal anti-DGKD antibody (ABIN7214554) specifically detects DGKD in WB, ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for DGKD antibody (AA 10-90) (ABIN7214554)

Target: DGKD (Diacylglycerol Kinase, delta 130kDa (DGKD))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGKD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-DGKD Antibody

Binding Specificity: AA 10-90

Purpose: Rabbit Anti-DGK-δ Polyclonal Antibody

Specificity: DGK-δ Polyclonal Antibody detects endogenous levels of DGK-δ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human DGK-delta at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DGKD

Target: DGKD (Diacylglycerol Kinase, delta 130kDa (DGKD))

Alternative Name: DGK-delta

Background: DGKD, KIAA0145, Diacylglycerol kinase delta, DAG kinase delta, 130 kDa diacylglycerol kinase, Diglyceride kinase delta, DGK-deltaDGKD encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms.

Molecular Weight: 135kD

Gene ID: 8527

UniProt: Q16760

Pathways: EGFR Signaling Pathway