DGKE antibody (AA 130-210)

Catalog No. ABIN7225514

This Rabbit Polyclonal antibody specifically detects DGKE in WB, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for DGKE antibody (AA 130-210) (ABIN7225514)

Target: DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGKE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DGKE Antibody

Binding Specificity: AA 130-210

Purpose: DGK-ε Polyclonal Antibody

Specificity: DGK-ε Polyclonal Antibody detects endogenous levels of DGK-ε protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human DGK-epsilon at AA range: 130-210

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for DGKE

Target: DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))

Alternative Name: DGK-epsilon

Background: Rabbit Anti-DGK-ε Polyclonal Antibody,DGKE, DAGK5, Diacylglycerol kinase epsilon, DAG kinase epsilon, Diglyceride kinase epsilon, DGK-epsilon,Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.,Diacylglycerol kinase epsilon

Molecular Weight: observerd band 60kDa

Gene ID: 8526

UniProt: P52429