DKK1 antibody (C-Term)

Catalog No. ABIN7218628

The Rabbit Polyclonal anti-DKK1 antibody (ABIN7218628) specifically detects DKK1 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for DKK1 antibody (C-Term) (ABIN7218628)

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DKK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-DKK1 Antibody

Binding Specificity: C-Term

Purpose: Rabbit Anti-Dkk-1 Polyclonal Antibody

Specificity: Dkk-1 Polyclonal Antibody detects endogenous levels of Dkk-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Dkk-1

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DKK1

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Alternative Name: Dkk-1

Background: DKK1, Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SKDKK1 encodes dickkopf WNT signaling pathway inhibitor 1 that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Molecular Weight: 29kD

Gene ID: 22943

UniProt: O94907

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation