AKR1C1 antibody (AA 30-110)

Catalog No. ABIN7219994

The Rabbit Polyclonal anti-AKR1C1 antibody is suitable to detect AKR1C1 in samples from Human. It has been validated for IHC, ELISA and IF.

Quick Overview for AKR1C1 antibody (AA 30-110) (ABIN7219994)

Target: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKR1C1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-AKR1C1 Antibody

Binding Specificity: AA 30-110

Purpose: Rabbit Anti-DD1 Polyclonal Antibody

Specificity: DD1 Polyclonal Antibody detects endogenous levels of DD1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human DD1 at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for AKR1C1

Target: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Alternative Name: DD1

Background: AKR1C1, DDH, DDH1, Aldo-keto reductase family 1 member C1, 20-alpha-hydroxysteroid dehydrogenase, 20-alpha-HSD, Chlordecone reductase homolog HAKRC, Dihydrodiol dehydrogenase 1/2, DD1/DD2, High-affinity hepatic bile acid-binding protein, HBAKR1C1 encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Molecular Weight: 37kD

Gene ID: 1645

UniProt: Q04828

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process