DMGDH antibody (AA 790-870)

Catalog No. ABIN7214582

The Rabbit Polyclonal anti-DMGDH antibody is suitable to detect DMGDH in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for DMGDH antibody (AA 790-870) (ABIN7214582)

Target: DMGDH (Dimethylglycine Dehydrogenase (DMGDH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DMGDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-DMGDH Antibody

Binding Specificity: AA 790-870

Purpose: Rabbit Anti-DMGDH Polyclonal Antibody

Specificity: DMGDH Polyclonal Antibody detects endogenous levels of DMGDH protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human DMGDH at AA range: 790-870

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DMGDH

Target: DMGDH (Dimethylglycine Dehydrogenase (DMGDH))

Alternative Name: DMGDH

Background: DMGDH, Dimethylglycine dehydrogenase, mitochondrial, ME2GLYDHDMGDH encodes an enzyme involved in the catabolism of choline, catalyzing the oxidative demethylation of dimethylglycine to form sarcosine. The enzyme (dimethylglycine dehydrogenase) is found as a monomer in the mitochondrial matrix, and uses flavin adenine dinucleotide and folate as cofactors. Mutation in this gene causes dimethylglycine dehydrogenase deficiency, characterized by a fishlike body odor, chronic muscle fatigue, and elevated levels of the muscle form of creatine kinase in serum. Alternative splicing results in multiple transcript variants.

Molecular Weight: 97kD

Gene ID: 29958

UniProt: Q9UI17