DAAM1 antibody (AA 400-500)

Catalog No. ABIN7228144

The Rabbit Polyclonal anti-DAAM1 antibody (ABIN7228144) specifically detects DAAM1 in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for DAAM1 antibody (AA 400-500) (ABIN7228144)

Target: DAAM1 (Dishevelled Associated Activator of Morphogenesis 1 (DAAM1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAAM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DAAM1 Antibody

Binding Specificity: AA 400-500

Purpose: Rabbit Anti-DAAM1 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of DAAM1

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human DAAM1 protein at AA range: 400-500

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:10000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DAAM1

Target: DAAM1 (Dishevelled Associated Activator of Morphogenesis 1 (DAAM1))

Alternative Name: DAAM1

Background: Disheveled-associated activator of morphogenesis 1Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by DAAM1 contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by DAAM1 is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct proteins.

Molecular Weight: 123kD

Gene ID: 23002

UniProt: Q9Y4D1

Pathways: WNT Signaling