RPA14 antibody (AA 30-80)

Catalog No. ABIN7231134

The Rabbit Polyclonal anti-RPA14 antibody (ABIN7231134) specifically detects RPA14 in WB and IHC. The antibody is reactive with Human and Mouse samples.

Quick Overview for RPA14 antibody (AA 30-80) (ABIN7231134)

Target: RPA14 (DNA-directed RNA polymerase I subunit RPA14 (RPA14))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RPA14 Antibody

Binding Specificity: AA 30-80

Purpose: Rabbit Anti-RPA14 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of RPA14

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human RPA14 protein at AA range: 30-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RPA14

Target: RPA14 (DNA-directed RNA polymerase I subunit RPA14 (RPA14))

Alternative Name: RPA14

Background: Replication protein A 14 kDa subunit, RP-A p14, Replication factor A protein 3, RF-A protein 3As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed: 9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. RPA3 (Replication Protein A3) is a Protein Coding gene. Diseases associated with RPA3 include Epidemic Typhus and Ovary Serous Adenocarcinoma. Among its related pathways are Mismatch repair and DNA Double-Strand Break Repair.

Molecular Weight: 14kD

Gene ID: 6119

UniProt: P35244