DOK4 antibody (AA 30-110)

Catalog No. ABIN7214608

The Rabbit Polyclonal anti-DOK4 antibody (ABIN7214608) specifically detects DOK4 in WB, IHC, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for DOK4 antibody (AA 30-110) (ABIN7214608)

Target: DOK4 (Docking Protein 4 (DOK4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-DOK4 Antibody

Binding Specificity: AA 30-110

Purpose: Rabbit Anti-Dok-4 Polyclonal Antibody

Specificity: Dok-4 Polyclonal Antibody detects endogenous levels of Dok-4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Dok-4 at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DOK4

Target: DOK4 (Docking Protein 4 (DOK4))

Alternative Name: Dok-4

Background: DOK4, Docking protein 4, Downstream of tyrosine kinase 4, Insulin receptor substrate 5, IRS-5, IRS5The downstream of kinase family (Dok-1-7) are members of a class of docking proteins that interact with receptor tyrosine kinases and, via this interaction, mediate biological responses within the body. Dok-4 (Downstream of kinase-4) is a 326 amino acid protein that contains one PH domain and one IRS-type PTB domain and belongs to the Dok family of interacting proteins. Expressed in a variety of tissues with highest expression in liver, heart, kidney and skeletal muscle, Dok-4 plays an important role in Ret-mediated neurite outgrowth and may link Ret with downstream effectors during neuronal differentiation. Additionally, Dok-4 is thought to play a positive role in the activation of MAPK pathways and may participate in T-cell induced immune system regulation. Overexpression of Dok-4 is associated with clear cell renal cell carcinoma, suggesting a role for Dok-4 in tumorigenesis.

Molecular Weight: 37kD

Gene ID: 55715

UniProt: Q8TEW6