DOK6 antibody (AA 80-160)

Catalog No. ABIN7214610

The Rabbit Polyclonal anti-DOK6 antibody is suitable to detect DOK6 in samples from Human and Mouse. It has been validated for WB, ELISA, IF and IHC.

Quick Overview for DOK6 antibody (AA 80-160) (ABIN7214610)

Target: DOK6 (Docking Protein 6 (DOK6))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-DOK6 Antibody

Binding Specificity: AA 80-160

Purpose: Rabbit Anti-Dok-6 Polyclonal Antibody

Specificity: Dok-6 Polyclonal Antibody detects endogenous levels of Dok-6 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Dok-6 at AA range: 80-160

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DOK6

Target: DOK6 (Docking Protein 6 (DOK6))

Alternative Name: Dok-6

Background: DOK6, DOK5L, Docking protein 6, Downstream of tyrosine kinase 6The downstream of kinase family (Dok-1-7) are members of a class of "docking" proteins that include the tyrosine kinase substrates IRS-1 and Cas, which contain multiple tyrosine residues and putative SH2 binding sites. Dok-4, Dok-5 and Dok-6 are more similar to each other than to the other Dok family members, and may constitute a subfamily of the DOK genes. Dok-5 is a tyrosine kinase substrate that enhances c-Ret-dependent activation of mitogen-activated protein kinase (MAPK). Dok-5 transcript is abundant in muscle and increases during T cell activation. Dok-5 protein undergoes tyrosine phosphorylation in response to insulin and insulin-like growth factor-1. Dok-6 is highly expressed in the developing central nervous system. It associates with Ret to transduce Ret-mediated processes such as axonal projection.

Molecular Weight: 43kD

Gene ID: 220164

UniProt: Q6PKX4