DOK2 antibody

Catalog No. ABIN7214604

The Rabbit Polyclonal anti-DOK2 antibody (ABIN7214604) specifically detects DOK2 in WB, ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for DOK2 antibody (ABIN7214604)

Target: DOK2

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-DOK2 Antibody

Purpose: Rabbit Anti-Dok-2 Polyclonal Antibody

Specificity: Dok-2 Polyclonal Antibody detects endogenous levels of Dok-2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Dok-2 around the non-phosphorylation site of Y299

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DOK2

Target: DOK2

Alternative Name: Dok-2

Background: DOK2, Docking protein 2, Downstream of tyrosine kinase 2, p56(dok-2)Docking protein 2 encoded by DOK2 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210 (bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 2 binds p120 (RasGAP) from CML cells.

Molecular Weight: 46kD

Gene ID: 9046

UniProt: O60496