DOT1L antibody (AA 700-780)

Catalog No. ABIN7228274

This Rabbit Polyclonal antibody specifically detects DOT1L in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for DOT1L antibody (AA 700-780) (ABIN7228274)

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOT1L antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DOT1L Antibody

Binding Specificity: AA 700-780

Purpose: DOT1L Polyclonal Antibody

Specificity: The antibody detects endogenous levels of DOT1L protein

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human DOT1L protein at AA range: 700-780

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for DOT1L

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Alternative Name: DOT1L

Background: Rabbit Anti-DOT1L Polyclonal Antibody,Histone-lysine N-methyltransferase, H3 lysine-79 specific, DOT1-like protein, Histone H3-K79 methyltransferase, H3-K79-HMTase, Lysine N-methyltransferase 4,DOT1L encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. DOT1L (DOT1 Like Histone Lysine Methyltransferase) is a Protein Coding gene. Diseases associated with DOT1L include Diffuse Gastric Cancer and Leukemia, Acute Myeloid. Among its related pathways are Chromatin organization and Transcriptional misregulation in cancer.,DOT1L

Molecular Weight: observerd band 191kDa

Gene ID: 84444

UniProt: Q8TEK3