DCLK2 antibody (AA 1-80)

Catalog No. ABIN7214526

The Rabbit Polyclonal anti-DCLK2 antibody (ABIN7214526) specifically detects DCLK2 in WB, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for DCLK2 antibody (AA 1-80) (ABIN7214526)

Target: DCLK2 (Doublecortin-Like Kinase 2 (DCLK2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCLK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-DCLK2 Antibody

Binding Specificity: AA 1-80

Purpose: Rabbit Anti-DCAMKL2 Polyclonal Antibody

Specificity: DCAMKL2 Polyclonal Antibody detects endogenous levels of DCAMKL2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human DCAMKL2 at AA range: 1-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DCLK2

Target: DCLK2 (Doublecortin-Like Kinase 2 (DCLK2))

Alternative Name: DCAMKL2

Background: DCLK2, DCAMKL2, DCDC3B, DCK2, Serine/threonine-protein kinase DCLK2, CaMK-like CREB regulatory kinase 2, CL2, CLICK-II, CLICK2, Doublecortin domain-containing protein 3B, Doublecortin-like and CAM kinase-like 2, Doublecortin-like kinase 2DCLK2 encodes a member of the protein kinase superfamily and the doublecortin family. Doublecortin like kinase 2 encoded by DCLK2 contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and DCLK2 share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified.

Molecular Weight: 83kD

Gene ID: 166614

UniProt: Q8N568