DAPP1 antibody

Catalog No. ABIN7213762

The Rabbit Polyclonal anti-DAPP1 antibody is suitable to detect DAPP1 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for DAPP1 antibody (ABIN7213762)

Target: DAPP1 (Dual Adaptor of Phosphotyrosine and 3-phosphoinositides (DAPP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAPP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-DAPP1 Antibody

Purpose: Rabbit Anti-BAM32 Polyclonal Antibody

Specificity: BAM32 Polyclonal Antibody detects endogenous levels of BAM32 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human BAM32 around the non-phosphorylation site of Y139

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DAPP1

Target: DAPP1 (Dual Adaptor of Phosphotyrosine and 3-phosphoinositides (DAPP1))

Alternative Name: BAM32

Background: DAPP1, BAM32, HSPC066, Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide, hDAPP1, B lymphocyte adapter protein Bam32, B-cell adapter molecule of 32 kDaB cell adapter molecule (BAM32) is also designated dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide (DAPP1) or B lymphocyte adapter protein. BAM32 is a B cell-associated adapter that is crucial for B cell antigen receptor signaling regulation. BAM32 interacts with Ptdlns and PLC g2 and, upon B cell activation, the protein is phosphorylated on tyrosine residues. It is a mainly cytoplasmic protein that can translocate to the cell membrane after cell stimulation. BAM32, which contains one PH domain and one SH2 domain, is primarily expressed in placenta and lung tissues, but can also be detected in heart, liver, pancreas and brain.

Molecular Weight: 32kD

Gene ID: 27071

UniProt: Q9UN19

Pathways: BCR Signaling