MKP-1/2 antibody (pSer296, pSer318)

Catalog No. ABIN7213286

The Rabbit Polyclonal anti-MKP-1/2 antibody (ABIN7213286) specifically detects MKP-1/2 in IHC, WB, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MKP-1/2 antibody (pSer296, pSer318) (ABIN7213286)

Target: MKP-1/2 (DUSP1/4) (Dual Specificity Phosphatase 1/4 (DUSP1/4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MKP-1/2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-MKP-1/2 Antibody

Binding Specificity: pSer296, pSer318

Purpose: Rabbit Anti-MKP-1/2 (phospho Ser296/318) Polyclonal Antibody

Specificity: Phospho-MKP-1/2 (S296/318) Polyclonal Antibody detects endogenous levels of MKP-1/2 protein only when phosphorylated at S296/318.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human MKP-1/2 Phospho-Ser296/318

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MKP-1/2

Target: MKP-1/2 (DUSP1/4) (Dual Specificity Phosphatase 1/4 (DUSP1/4))

Alternative Name: MKP-1/2

Background: DUSP1, CL100, MKP1, PTPN10, VH1, Dual specificity protein phosphatase 1, Dual specificity protein phosphatase hVH1, Mitogen-activated protein kinase phosphatase 1, MAP kinase phosphatase 1, MKP-1, Protein-tyrosine phosphatase CL100, DUSP4,The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.

Molecular Weight: 45kD

Gene ID: 1843, 1846

UniProt: P28562, Q13115