Dynamitin antibody (AA 310-390)

Catalog No. ABIN7214636

This Rabbit Polyclonal antibody specifically detects Dynamitin in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Dynamitin antibody (AA 310-390) (ABIN7214636)

Target: Dynamitin (DCTN2) (Dynactin 2 (p50) (DCTN2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dynamitin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Dynamitin Antibody

Binding Specificity: AA 310-390

Purpose: Rabbit Anti-Dynactin 2 Polyclonal Antibody

Specificity: Dynactin 2 Polyclonal Antibody detects endogenous levels of Dynactin 2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Dynactin 2 at AA range: 310-390

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Dynamitin

Target: Dynamitin (DCTN2) (Dynactin 2 (p50) (DCTN2))

Alternative Name: Dynactin 2

Background: DCTN2, DCTN50, Dynactin subunit 2, 50 kDa dynein-associated polypeptide, Dynactin complex 50 kDa subunit, DCTN-50, p50 dynamitinDCTN2 encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for DCTN2.

Molecular Weight: 50kD

Gene ID: 10540

UniProt: Q13561

Pathways: M Phase, Ribonucleoprotein Complex Subunit Organization