Dynamin 1 antibody (pSer774)

Catalog No. ABIN7220058

The Rabbit Polyclonal anti-Dynamin 1 antibody is suitable to detect Dynamin 1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for Dynamin 1 antibody (pSer774) (ABIN7220058)

Target: Dynamin 1 (DNM1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dynamin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Dynamin 1 Antibody

Binding Specificity: pSer774

Purpose: Rabbit Anti-Dynamin I (phospho Ser774) Polyclonal Antibody

Specificity: Phospho-Dynamin I (S774) Polyclonal Antibody detects endogenous levels of Dynamin I protein only when phosphorylated at S774.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Dynamin I Phospho-Ser774

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Dynamin 1

Target: Dynamin 1 (DNM1)

Alternative Name: Dynamin I

Background: DNM1, DNM, Dynamin-1DNM1 encodes a member of the dynamin subfamily of GTP-binding proteins. Dynamin-1 possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.

Molecular Weight: 97kD

Gene ID: 1759

UniProt: Q05193

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling