DKC1 antibody (AA 140-220)

Catalog No. ABIN7220050

This Rabbit Polyclonal antibody specifically detects DKC1 in WB, IF, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for DKC1 antibody (AA 140-220) (ABIN7220050)

Target: DKC1 (Dyskeratosis Congenita 1, Dyskerin (DKC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DKC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DKC1 Antibody

Binding Specificity: AA 140-220

Purpose: Dyskerin Polyclonal Antibody

Specificity: Dyskerin Polyclonal Antibody detects endogenous levels of Dyskerin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Dyskerin at AA range: 140-220

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for DKC1

Target: DKC1 (Dyskeratosis Congenita 1, Dyskerin (DKC1))

Alternative Name: Dyskerin

Background: Rabbit Anti-Dyskerin Polyclonal Antibody,DKC1, NOLA4, H/ACA ribonucleoprotein complex subunit 4, CBF5 homolog, Dyskerin, Nopp140-associated protein of 57 kDa, Nucleolar protein NAP57, Nucleolar protein family A member 4, snoRNP protein DKC1,Dihydroorotate dehydrogenase encoded by DHODH catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. DKC1 functions in two distinct complexes. It plays an active role in telomerase stabilization and maintenance, as well as recognition of snoRNAs containing H/ACA sequences which provides stability during biogenesis and assembly into H/ACA small nucleolar RNA ribonucleoproteins (snoRNPs). This gene is highly conserved and widely expressed, and may play additional roles in nucleo-cytoplasmic shuttling, DNA damage response, and cell adhesion. Mutations have been associated with X-linked dyskeratosis congenita. Alternative splicing results in multiple transcript variants.,H

Molecular Weight: observerd band 57kDa

Gene ID: 1736

UniProt: O60832

Pathways: Telomere Maintenance