E2F4/E2F5 (E2F4/5) (AA 30-110) antibody

Catalog No. ABIN7214648

The Rabbit Polyclonal anti-E2F4/E2F5 antibody (ABIN7214648) specifically detects E2F4/E2F5 in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for E2F4/E2F5 (E2F4/5) (AA 30-110) antibody (ABIN7214648)

Target: E2F4/E2F5 (E2F4/5)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details

Binding Specificity: AA 30-110

Purpose: Rabbit Anti-E2F-4/5 Polyclonal Antibody

Specificity: E2F-4/5 Polyclonal Antibody detects endogenous levels of E2F-4/5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human E2F-4/5 at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: E2F4/E2F5 (E2F4/5)

Alternative Name: E2F-4/5

Background: E2F4, Transcription factor E2F4, E2F-4, E2F5, Transcription factor E2F5, E2F-5E2F transcription factor 4 encoded by E2F4 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.

Molecular Weight: 44kD

Gene ID: 1874, 1875

UniProt: Q16254, Q15329

Pathways: Mitotic G1-G1/S Phases