RAD18 antibody (AA 270-350)

Catalog No. ABIN7216744

The Rabbit Polyclonal anti-RAD18 antibody (ABIN7216744) specifically detects RAD18 in WB and IHC. The antibody is reactive with Human and Rat samples.

Quick Overview for RAD18 antibody (AA 270-350) (ABIN7216744)

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RAD18 Antibody

Binding Specificity: AA 270-350

Purpose: Rabbit Anti-Rad18 Polyclonal Antibody

Specificity: Rad18 Polyclonal Antibody detects endogenous levels of Rad18 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Rad18 at AA range: 270-350

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RAD18

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Alternative Name: Rad18

Background: RAD18, RNF73, E3 ubiquitin-protein ligase RAD18, Postreplication repair protein RAD18, hHR18, hRAD18, RING finger protein 73RAD18, E3 ubiquitin protein ligase encoded by RAD18 is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, RAD18, E3 ubiquitin protein ligase is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Molecular Weight: 56kD

Gene ID: 56852

UniProt: Q9NS91