EFHD1 antibody

Catalog No. ABIN7203498

The Mouse Monoclonal anti-EFHD1 antibody is suitable to detect EFHD1 in samples from Human, Mouse and Rat. It has been validated for WB, IF and IHC.

Quick Overview for EFHD1 antibody (ABIN7203498)

Target: EFHD1 (EF-Hand Domain Family, Member D1 (EFHD1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This EFHD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-EFHD1 Antibody

Purpose: Mouse Anti-EFHD1 Monoclonal Antibody

Specificity: The antibody detects endogenous EFHD1 proteins.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: Synthetic Peptide

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:2000,IF 1:100-200,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.5 % BSA, 0.02 % sodium azide as Preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EFHD1

Target: EFHD1 (EF-Hand Domain Family, Member D1 (EFHD1))

Alternative Name: EFHD1

Background: EFHD1 encodes a member of the EF-hand super family of calcium binding proteins, which are involved in a variety of cellular processes including mitosis, synaptic transmission, and cytoskeletal rearrangement. EF-hand domain family member D1 encoded by EFHD1 is composed of an N-terminal disordered region, proline-rich elements, two EF-hands, and a C-terminal coiled-coil domain. EF-hand domain family member D1 has been shown to associate with the mitochondrial inner membrane, and in HeLa cells, acts as a novel mitochondrial calcium ion sensor for mitochondrial flash activation. Alternative splicing results in multiple transcript variants.

Molecular Weight: 27kD

Gene ID: 80303

UniProt: Q9BUP0