KLF1 antibody (AA 280-360)

Catalog No. ABIN7214718

The Rabbit Polyclonal anti-KLF1 antibody (ABIN7214718) specifically detects KLF1 in ELISA, WB and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for KLF1 antibody (AA 280-360) (ABIN7214718)

Target: KLF1 (Kruppel-Like Factor 1 (erythroid) (KLF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-KLF1 Antibody

Binding Specificity: AA 280-360

Purpose: Rabbit Anti-EKLF/CKLF/UKLF Polyclonal Antibody

Specificity: EKLF/CKLF/UKLF Polyclonal Antibody detects endogenous levels of EKLF/CKLF/UKLF protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human EKLF/CKLF/UKLF at AA range: 280-360

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for KLF1

Target: KLF1 (Kruppel-Like Factor 1 (erythroid) (KLF1))

Alternative Name: EKLF/CKLF/UKLF

Background: KLF1, EKLF, Krueppel-like factor 1, Erythroid krueppel-like transcription factor, EKLF, KLF5, BTEB2, CKLF, IKLF, Krueppel-like factor 5, Basic transcription element-binding protein 2, BTE-binding protein 2, Colon krueppel-like factor, GC-boKLF1 encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in KLF1 result in the dominant In (Lu) blood phenotype.

Molecular Weight: 38kD 20kD

Gene ID: 10661, 688, 8609

UniProt: Q13351, Q13887, O75840