Elongin A1 antibody (AA 100-180)

Catalog No. ABIN7214724

The Rabbit Polyclonal anti-Elongin A1 antibody is suitable to detect Elongin A1 in samples from Human, Mouse and Rat. It has been validated for ELISA, IHC, WB and IF.

Quick Overview for Elongin A1 antibody (AA 100-180) (ABIN7214724)

Target: Elongin A1

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Elongin A1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Elongin A1 Antibody

Binding Specificity: AA 100-180

Purpose: Rabbit Anti-Elongin A1 Polyclonal Antibody

Specificity: Elongin A1 Polyclonal Antibody detects endogenous levels of Elongin A1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Elongin A1 at AA range: 100-180

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Elongin A1

Target: Elongin A1

Background: TCEB3, MSTP059, Transcription elongation factor B polypeptide 3, Elongin 110 kDa subunit, Elongin-A, EloA, RNA polymerase II transcription factor SIII subunit A1, SIII p110TCEB3 encodes the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.

Molecular Weight: 87kD

Gene ID: 6924

UniProt: Q14241