Endonuclease G antibody

Catalog No. ABIN7230168

The Rabbit Polyclonal anti-Endonuclease G antibody (ABIN7230168) specifically detects Endonuclease G in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for Endonuclease G antibody (ABIN7230168)

Target: Endonuclease G (ENDOG)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endonuclease G antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Endonuclease G Antibody

Purpose: Rabbit Anti-NUCG Polyclonal Antibody

Specificity: The antibody detects endogenous levels of NUCG protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human NUCG protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Endonuclease G

Target: Endonuclease G (ENDOG)

Alternative Name: NUCG

Background: Endonuclease G, mitochondrial, Endo GCleaves DNA at double-stranded (DG)n. DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA (By similarity).

Molecular Weight: 32kD

Gene ID: 2021

UniProt: Q14249

Pathways: Apoptosis