EHHADH antibody (AA 450-530)

Catalog No. ABIN7216468

This Rabbit Polyclonal antibody specifically detects EHHADH in WB, ELISA and IHC (p). It exhibits reactivity toward Human and Rat.

Quick Overview for EHHADH antibody (AA 450-530) (ABIN7216468)

Target: EHHADH (Enoyl-CoA, Hydratase/3-Hydroxyacyl CoA Dehydrogenase (EHHADH))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EHHADH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EHHADH Antibody

Binding Specificity: AA 450-530

Purpose: PBFE Polyclonal Antibody

Specificity: PBFE Polyclonal Antibody detects endogenous levels of PBFE protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human PBFE at AA range: 450-530

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for EHHADH

Target: EHHADH (Enoyl-CoA, Hydratase/3-Hydroxyacyl CoA Dehydrogenase (EHHADH))

Alternative Name: PBFE

Background: Rabbit Anti-PBFE Polyclonal Antibody,EHHADH, ECHD, Peroxisomal bifunctional enzyme, PBE, PBFE,Enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase by EHHADH is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in EHHADH are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for EHHADH.,Peroxisomal bifunctional enzyme

Molecular Weight: observerd band 80kDa

Gene ID: 1962

UniProt: Q08426

Pathways: Monocarboxylic Acid Catabolic Process