EPHB1/2 antibody (pTyr594, pTyr604)

Catalog No. ABIN7213198

The Rabbit Polyclonal anti-EPHB1/2 antibody has been validated for IF, WB and ELISA. It is suitable to detect EPHB1/2 in samples from Human, Mouse and Rat.

Quick Overview for EPHB1/2 antibody (pTyr594, pTyr604) (ABIN7213198)

Target: EPHB1/2 (EPH Receptor B1/2 (EPHB1/2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPHB1/2 antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), ELISA

Product Details anti-EPHB1/2 Antibody

Binding Specificity: pTyr594, pTyr604

Purpose: EphB1/2 (phospho Tyr594/604) Polyclonal Antibody

Specificity: Phospho-EphB1/2 (Y594/604) Polyclonal Antibody detects endogenous levels of EphB1/2 protein only when phosphorylated at Y594/604.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human EphB1/2 Phospho-Tyr594/604

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for EPHB1/2

Target: EPHB1/2 (EPH Receptor B1/2 (EPHB1/2))

Alternative Name: EphB1/2

Background: Rabbit Anti-EphB1/2 (phospho Tyr594/604) Polyclonal Antibody,EPHB1, ELK, EPHT2, HEK6, NET, Ephrin type-B receptor 1, ELK, EPH tyrosine kinase 2, EPH-like kinase 6, EK6, hEK6, Neuronally-expressed EPH-related tyrosine kinase, NET, Tyrosine-protein kinase receptor EPH-2, EPHB2, DRT, EPHT3, EPTH3, ERK,Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family.EPH receptor B1 encoded by EPHB1 is a receptor for ephrin-B family members.,Ephrin type-B receptor 1

Molecular Weight: observerd band 110kDa

Gene ID: 2047, 1969