EPHB1/2 antibody

Catalog No. ABIN7214764

The Rabbit Polyclonal anti-EPHB1/2 antibody (ABIN7214764) specifically detects EPHB1/2 in IF, WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for EPHB1/2 antibody (ABIN7214764)

Target: EPHB1/2 (EPH Receptor B1/2 (EPHB1/2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPHB1/2 antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-EPHB1/2 Antibody

Purpose: Rabbit Anti-EphB1/2 Polyclonal Antibody

Specificity: EphB1/2 Polyclonal Antibody detects endogenous levels of EphB1/2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human EphB1/2 around the non-phosphorylation site of Y594/604

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EPHB1/2

Target: EPHB1/2 (EPH Receptor B1/2 (EPHB1/2))

Alternative Name: EphB1/2

Background: EPHB1, ELK, EPHT2, HEK6, NET, Ephrin type-B receptor 1, ELK, EPH tyrosine kinase 2, EPH-like kinase 6, EK6, hEK6, Neuronally-expressed EPH-related tyrosine kinase, NET, Tyrosine-protein kinase receptor EPH-2, EPHB2, DRT, EPHT3, EPTH3, ERK,Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family.EPH receptor B1 encoded by EPHB1 is a receptor for ephrin-B family members.

Molecular Weight: 110kD

Gene ID: 2047, 1969

UniProt: P54762, P29323