EPHB1/2 antibody (pTyr594, pTyr604)

Catalog No. ABIN1531587

The Rabbit Polyclonal anti-EPHB1/2 antibody (ABIN1531587) specifically detects EPHB1/2 in IF, WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for EPHB1/2 antibody (pTyr594, pTyr604) (ABIN1531587)

Target: EPHB1/2 (EPH Receptor B1/2 (EPHB1/2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPHB1/2 antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), ELISA

Product Details anti-EPHB1/2 Antibody

Binding Specificity: AA 561-610, pTyr594, pTyr604

Purpose: EphB1/2 (Phospho-Tyr594/596) Antibody

Specificity: Phospho-EphB1/2 (Y594/604) Polyclonal Antibody detects endogenous levels of EphB1/2 protein only when phosphorylated at Y594/604.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human EPHB1/2 around the phosphorylation site of Tyr594/604. AA range:561-610

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for EPHB1/2

Target: EPHB1/2 (EPH Receptor B1/2 (EPHB1/2))

Alternative Name: EPHB1/2

Background:

Synonyms: EPHB1, ELK, EPHT2, HEK6, NET, Ephrin type-B receptor 1, ELK, EPH tyrosine kinase 2, EPH-like kinase 6, EK6, hEK6, Neuronally-expressed EPH-related tyrosine kinase, NET, Tyrosine-protein kinase receptor EPH-2, EPHB2, DRT, EPHT3, EPTH3, ERK,

Background: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family.The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008],

Gene Name: EPHB1/EPHB2

Protein Name: Ephrin type-B receptor 1/2

Molecular Weight: 109 kDa

Gene ID: 2047, 1969

OMIM: 600600

UniProt: P54762