EPH Receptor B4 antibody (AA 540-620)

Catalog No. ABIN7220308

The Rabbit Polyclonal anti-EPH Receptor B4 antibody is suitable to detect EPH Receptor B4 in samples from Human and Mouse. It has been validated for WB, ELISA, IF and IHC (p).

Quick Overview for EPH Receptor B4 antibody (AA 540-620) (ABIN7220308)

Target: EPH Receptor B4 (EPHB4)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor B4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EPH Receptor B4 Antibody

Binding Specificity: AA 540-620

Purpose: EphB4 Polyclonal Antibody

Specificity: EphB4 Polyclonal Antibody detects endogenous levels of EphB4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human EphB4 at AA range: 540-620

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for EPH Receptor B4

Target: EPH Receptor B4 (EPHB4)

Alternative Name: EphB4

Background: Rabbit Anti-EphB4 Polyclonal Antibody,EPHB4, HTK, MYK1, TYRO11, Ephrin type-B receptor 4, Hepatoma transmembrane kinase, Tyrosine-protein kinase TYRO11,Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The EPH receptor B4 encoded by EPHB4 binds to ephrin-B2 and plays an essential role in vascular development.,Ephrin type-B receptor 4

Molecular Weight: observerd band 108kDa

Gene ID: 2050

UniProt: P54760

Pathways: RTK Signaling