ERLIN1/2 antibody (AA 31-80)

Catalog No. ABIN7226896

The Rabbit Polyclonal anti-ERLIN1/2 antibody is suitable to detect ERLIN1/2 in samples from Human, Mouse and Rat. It has been validated for ELISA and WB.

Quick Overview for ERLIN1/2 antibody (AA 31-80) (ABIN7226896)

Target: ERLIN1/2

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERLIN1/2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-ERLIN1/2 Antibody

Binding Specificity: AA 31-80

Purpose: Rabbit Anti-ERLIN1/2 Polyclonal Antibody

Specificity: ERLIN1/2 Polyclonal Antibody detects endogenous levels of ERLIN1/2.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from ERLIN1/2 at AA range: 31-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:10000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ERLIN1/2

Target: ERLIN1/2

Background: Erlin-1 and erlin-2 (SPFH1 and SPFH2) are SPFH domain-containing proteins that belong to the prohibitin family. The N-termal domain of erlin proteins contains ER-targeting sequences responsible for their translocation to the endoplasmic reticulum (ER). In the ER, erlin-1 and erlin-2 specifically associate with the detergent resistant lipid raft microdomain of the membrane. Erlin-1 may be involved in dentritic cell activation and erlin-2 has been shown to regulate the ER-associated degradation (ERAD) pathway by interacting with endogenous substrates and resulting in their polyubiquitination and degradation.

Molecular Weight: 40kD,42kD

UniProt: O75477, O94905