ERF antibody (pThr526)

Catalog No. ABIN7220372

The Rabbit Polyclonal anti-ERF antibody is suitable to detect ERF in samples from Human and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for ERF antibody (pThr526) (ABIN7220372)

Target: ERF (Ets2 Repressor Factor (ERF))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-ERF Antibody

Binding Specificity: pThr526

Purpose: Rabbit Anti-ERF (phospho Thr526) Polyclonal Antibody

Specificity: Phospho-ERF (T526) Polyclonal Antibody detects endogenous levels of ERF protein only when phosphorylated at T526.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human ERF Phospho-Thr526

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ERF

Target: ERF (Ets2 Repressor Factor (ERF))

Alternative Name: ERF

Background: ERF, ETS domain-containing transcription factor ERF, Ets2 repressor factor, PE-2ETS2 is a transcription factor and protooncogene involved in development, apoptosis, and the regulation of telomerase. The protein encoded by ERF binds to the ETS2 promoter and is a strong repressor of ETS2 transcription. Several transcript variants encoding two different isoforms have been found for this gene.

Molecular Weight: 58kD

Gene ID: 2077

UniProt: P50548